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The hCTR cells were cultured in growth medium consisting of Dulbeccos Modified Eagles Medium (DMEM, Gibco, #31966-021) supplemented with 10% v/v foetal bovine serum (FBS, Gibco, #16140-071), 1% v/v Penicillin-Streptomycin (Gibco, #15140-122), 0.6 mg/mL Geneticin G418 (Gibco, #10131-027) and 0.2 mg/mL Hygromycin (Invitrogen, #10687010)

Melnyk S, Pogribna M, Pogribny IP, Yi P, and James SJ (2000)

[DOI] [PMC free article] [PubMed] [Google Scholar] 152.Herbert B.M., Blechert J., Hautzinger M., Matthias E., Herbert C

doi: 10.2174/1566524022666220525144630 72 MaLHeYXieHWangJChenJSongSet al

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Together these findings, therefore, suggest that alsin protects cells from oxidative stress and could be a redox sensing protein, similar to Rab5, Rac1, and SOD1