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PMID: 171 LooRLChanQNicholsonJKHolmesE

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For immunoprecipitation, HEK293T cells expressing GFP or HA-tagged proteins were lysed with mild immunoprecipitation buffer containing 150 mM NaCl, 50 mM Tris-HCl, pH 7.5, 0.5% NP40, 1 mM PMSF, protease inhibitor cocktail (Sigma Aldrich), mixed with 10 L GFP-trap or HA antibody conjugated agarose, and incubated for 4 h in cold room with end to end rotation

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Cai, R., Qin, B., Chen, Y., Zhang, L., Yang, R., Chen, S., & Wang, W