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48 h after transfection, cells were washed twice with pre-warmed PBS and fixed with 4% paraformaldehyde (PFA) for 1015 min

J Biol Chem 281:1742017431 Michelet F, Gueguen R, Leroy P, Wellman M, Nicolas A, Siest G (1995) Blood and plasma glutathione measured in healthy subjects by HPLC: relation to sex, aging, biological variables, and life habits

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Phthalates, e.g., benzyl butyl phthalate (BBP), dibutyl phthalate (DPB), and di(2-ethylhexyl) phthalate (DEHP), are known to have adverse health effects 17 , but they have nonetheless been found in various everyday plastic products, including food contact materials 18 and medical supplies 19

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Storing in the original carton or in the back of the refrigerator (less light exposure) is the standard