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Bone-marrow cells were stained with the antibody mixture (lineage cocktail (Mac1 (CD11b), Gr1 (Ly-6G/C), Ter119 (Ly-76), CD3, CD4, CD8a (Ly-2), and B220 (CD45R)), c-Kit, Sca1, CD48 and CD150 antibodies) (BioLegend, 101226, 108424, 116223, 100222, 100414, 100714, 103224, 105812, 108120, 103404 and 115904) for 20 min at 4 C, washed and analyzed by flow cytometry

For tissue clearing, the sectioned small intestine was incubated in 4% PFA at 4 C with shaking overnight and washed twice with 1 PBS for ~ 2 h at room temperature

Promotes cell renewal and reduces fine lines

The Signaling Stack: 24K Gold| Methylene Blue | GHK-Cu | SNAP-8 | Matrixyl 3000 | Tripeptide-29

Using publicly available single cell RNAseq datasets from both mouse and human studies we investigated the distribution of the genes for MT1 ( Mtnr1a ) and MT2 ( Mtnr1b ) across multiple organ systems

10.3389/fneur.2017.00333 93