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Rhonda Patrick: We injected those into the muscle tissue of worms

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Pharmacological advancements in bile acid transport include ASBT inhibitors, which reduce bile acid reabsorption, and MRP2 activator which enhance bilirubin excretion [54]

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Forty male, 6-week-old, specific pathogen-free C57BL/6 mice (Mus musculus) were purchased from the Jackson Laboratory (Bar Harbor, ME, USA) and transferred to the animal facility at the University of Maine (Orono, ME, USA)

The other consistent context in the preclinical literature is a metabolic environment the mouse studies pair the compound with diet/energy-balance conditions rather than testing it in isolation