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doi: 10.1016/j.nbd.2016.01.020 303 WooT.-G.YoonM.-H.KangS.-M.ParkS.ChoJ.-H.HwangY

Conclusion When administered in a reasonably safe dose and continually, over long periods of time, glutathione can be a safe drug to take

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1- Free GSH=DTNB+Sample+Substrate 2- Total GSH=DTNB+Sample+Substrate+ NADPH+glutathione reductase 3- GSSG=Total GSH-Free GSH Another method to measure GSSG separately, is to remove the free GSH from DTNB reaction process by modification of free GSH with N-ethylmaleimide (NEM) or vinylpyridine prior to conducting assay