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In this case, supporting small and sustainable changes to lifestyle can still yield important improvements, but additional therapeutics are often still necessary to address the detrimental chronic presence of NAFLD
For fluorescence microscopy studies, HL-60 cells and HepG2 cells (seeding densities 1 10 4 cells/well and 8 10 4 cells/well, respectively) were placed in serum-free medium to facilitate their adhesion to coverslips and cultured on lysine-coated 8-well chamber slides (IBIDI) following the procedure detailed by 2.8 Cell surface binding, endocytosis, and downregulation of TfRs For surface binding and endocytosis of AF-labeled proteins, the cells were rinsed twice with ice-cold HEPES-buffered, serum-free media, pH 7.4, and incubated for 1 h on ice with either 500 nM hemeAF546HPX, hemeAF546HPX, or AF488 holo-human Tf
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It functions as a dual GIP/GLP-1 receptor agonist, meaning one peptide chain binds to and activates both the glucose-dependent insulinotropic polypeptide receptor and the GLP-1 receptor
