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In addition, the NMF levels were increased at 12 weeks compared with placebo-control levels

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It was previously available through compounding pharmacies as a bulk drug substance, but the FDA reclassified it as a Category 2 substance, which effectively restricts compounding pharmacies from producing it

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Extracts of the medium were incubated at 80 C for at least 15 min before being centrifuged at 15,000 g for 20 min

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