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Unless otherwise specified, all reactions were performed in 20 mM Tris-HCl pH 8.0,150 mM NaCl buffer, with 25 M of freshly prepared Th-T (ThermoFisher Scientific) added to each reaction

10.1186/s41231-019-0050-7 135 SixN.SeptierD.Chaussain-MillerC.BlacherR.DenBestenP.GoldbergM

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Storage temperature, time since mixing, and handling all affect how much active peptide remains in the later doses from a vial

Think of the glucagon receptor as a third tier, says Sue DeCotiis, MD, a triple-board certified weight loss specialist and a clinical instructor at the NYU Grossman School of Medicine in New York City