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The methods of Arnon 33 and Lichtenthaler 34 were employed to determine the content of chlorophyll and carotenoids, respectively

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Circulation 138(13):13301342 Jansweijer JA, Nieuwhof K, Russo F, Hoorntje ET, Jongbloed JD, Lekanne Deprez RH, Postma AV, Bronk M, van Rijsingen IA, de Haij S, Biagini E, van Haelst PL, van Wijngaarden J, van den Berg MP, Wilde AA, Mannens MM, de Boer RA, van Spaendonck-Zwarts KY, van Tintelen JP, Pinto YM (2017) Truncating titin mutations are associated with a mild and treatable form of dilated cardiomyopathy

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The PreScission Protease is GST-tagged and during elution the protease will remain bound to the medium providing high purity of the target protein