Once a peptide molecule unfolds, returning it to the correct temperature does not refold it
After incubation with the secondary antibody, the cells were washed with PBST and mounted using a Fluoroshield Mounting Medium with DAPI (Abcam ab104139)
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Preclinical research indicates it remains structurally intact in acidic and enzymatic environments, making it a reliable subject for degradation and interaction studies
Historically, hard-to-treat sites forced a step-up to injectable biologics