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Urine collected in the bladder is expelled through the urethra, which is longer in males than in females, and in both cases, the urethra connects with the genital organs, leading to the formation of the urogenital system
Subsequently, the HUVECs are stained with a cell-permeable dye calcein AM (Corning, catalog number 354216) for 30 min, and then observed for capillary-like tubule formation using an inverted microscope
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