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The agarose was then subjected to washing steps, including once with low salt buffer (20 mM Tris-HCl, pH 8.0, 150 mM NaCl, 0.1% SDS, 1% Triton X-100, 2 mM EDTA), high salt buffer (20 mM Tris-HCl, pH 8.0, 0.5 M NaCl, 0.1% SDS, 1% Triton X-100, 2 mM EDTA), LiCl buffer, and twice with TE buffer