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The macrovesicular and the microvesicular fatty change (vacuolation) generally located at the cell periphery stains positively for Oil Red-O and the membranes surrounding these lipid vacuoles stain positively for adipophilin (a protein that forms the membrane around non-lysosomal lipid droplets) but negative for LAMP-2 (a lysosome-associated protein) by immunohistochemical techniques (Obert et al
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Knocking out Nrf2 or using HO-1 antagonist reversed DOX-induced heart injury and death in mice, indicating that free iron released by heme degradation is a prerequisite for inducing heart injury
Commun Biol 5 , 1293 (2022)
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