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PCR was conducted in a thermal cycler with the following parameters: initial denaturation was at 95C for 5 min and was followed by 40 cycles of denaturation at 95C for 10 s and annealing and extension at 60C for 30 s

The frequency of Vitamin B12 injections varies from daily to monthly, depending on the treatment phase

Combining peptide treatment with approaches that increase hair thickness (like microneedling) may produce more satisfying visual results

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This method, designed for ease of use, is effective and straightforward