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Nonspecific binding was blocked by immersing the slides in 2% BSA in PBS for 90 min and incubating with background sniper (Biocare, #BS966) for 10 min

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Cagrilintide + Retatrutide Storage & Handling Storage Reference (Both Compounds) State Lyophilized (long-term) Cagrilintide -4 F (-20 C), 12+ months Retatrutide -4 F (-20 C), 12+ months State Lyophilized (short-term) Cagrilintide 35.6-46.4 F (2-8 C), several months Retatrutide 35.6-46.4 F (2-8 C), several months State Reconstituted (liquid) Cagrilintide 35.6-46.4 F (2-8 C), 28-30 days Retatrutide 35.6-46.4 F (2-8 C), 28-30 days State Color when reconstituted Cagrilintide Clear, colorless Retatrutide Clear, colorless State Shake