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Key milestones in Epitalon research compound development include: Initial isolation and animal studies using the pineal extract Epithalamin across multiple species, including mice, rats, and primates Confirmation through HPLC and mass spectrometry that AEDG exists naturally within the pineal complex Transition to synthetic Epitalon as a standardised research tool, enabling controlled dosing and reproducible experimental design Expansion of research beyond the original laboratory, including independent replication studies published from 2025 onward This progression from a crude biological extract to a defined synthetic compound is what allows modern Epitalon peptide research to apply rigorous, reproducible experimental protocols

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Multiple dose levels (1, 4, 8, 12 mg) were tested in Phase 2, with the Phase 3 dose selection to be refined based on efficacy-tolerability balance