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Consequently, the GSH deprotonation step was considered non-rate-determining and thermodynamically favoured, leading us to use the GSH-thiolate as the starting point to compute the reaction mechanism for the conversion of flav-3-on-4-ol ( 7 ) into cyanidin ( 8-B4 ) in the wild-type enzyme and the C13S variant (Extended Data Fig

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Hippocampal neurons (2 10 5 cells per square cm) were cultured from P1 Sprague Dawley rats on plates coated with poly-L-lysine from Sigma (St

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Gastrointestinal symptoms are always caused by changes in serum thyroid hormone, among which constipation is the most common clinical symptom

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