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Oral methionine compared with intravenous N-acetyl cysteine for paracetamol overdose"

Korean Med

Glutathione, a vital cellular antioxidant, plays a crucial role in maintaining cellular health

A total of 14 HPLC fractions were collected, separated from solvent in vacuum, the residue of each fraction was suspended in H 2 O (10 mL), freeze-dried twice and, then, screened for the target compounds showing the pseudomolecular ions m/z 453 and 837, respectively, by means of UPLC-TOF MS using the following chromatographic conditions (0.3 mL/min, 40 C): starting with a mixture of H 2 O/MeOH (40/60, v/v), the MeOH content was kept constant for 1 min at 60%, increased to 90% within 8 min, increased to 95% within 10 min, increased to 100% within 1 min, kept constant for 1 min, decreased within 1 min to 60% MeOH, followed by re-equilibration for 1 min