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Using higher doses

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Western blotting HTMCs were grown in the above-mentioned TMC medium in five 12-well plates at a density of 5 10 4 /ml for 24 h, and the medium was replaced with a new one, containing 1 l of water (negative control), TGF-1 (20 g/ml) or glucaric acid (10 mM) for 10 min

Assessment of lipid peroxidation by measuring malondialdehyde (MDA) and relatives in biological samples: Analytical and biological challenges

Glutathione helps to reduce oxidative stress in the liver by neutralizing these free radicals, thus decreasing inflammation and helping to control the progression of liver damage

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