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For in vivo studies, a minimum of five mice per group was selected, which has been shown to be sufficient to detect biologically and statistically significant differences in similar experiments

TUNEL studies A terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay was used to assess DNA fragmentation during apoptosis in the myocardium, using a commercial detection kit (Roche, Switzerland)

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116 As previously noted, in a randomized controlled trial in 468 men with infertility and sperm abnormalities, 600 mg NAC in combination with 200 mcg selenium daily improved semen quality, increased testosterone, LH, and inhibin B levels, and decreased FSH levels after 30 weeks

Aoyama K1, Nakaki T

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