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Any injection carries a risk of infection, particularly when these are not purified because theyre not cleared for human use

doi:10.1002/nbm.2772, PMID: 22281935 Puri BK, Agour M, Gunatilake KD, Fernando KA, Gurusinghe AI, Treasaden IH (2009) An in vivo proton neurospectroscopy study of cerebral oxidative stress in myalgic encephalomyelitis (chronic fatigue syndrome)

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The outer skin may be pulled away from the muscle tissue to ensure the needle does not pierce the muscle