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For experiments involving alterations to the culture medium, cells were seeded in regular RPMI 1640 with 5% dialysed FBS (Gibco, 26400044) and 1% penicillin and streptomycin

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Spare respiratory capacity was calculated as the difference between the basal OCR and the maximal OCR after the addition of FCCP

, Meharry Medical College 40+ years of clinical experience Dr

Put together, these factors make GHB less visible, but potentially more accessible than many parents expect