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Consequently, we have meticulously updated and comprehensively rearticulated these two mechanisms, with a particular focus on innovative and all-encompassing methodologies (Figure 2)

Following blocking with 5% nonfat dry milk in TBST buffer (0.01 m Tris-HCl, pH 8.0, 0.15 m NaCl, and 0.1% Tween 20), membranes were immunoblotted with different primary antibodies