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In some specific research protocols, alternatives are used: Sterile water for injection (WFI) , when the entire contents of the vial are used at once and a preservative is not needed Physiological saline (0.9% NaCl) , in some applications requiring an isotonic environment Acidic solvents (e.g

Even a capped vial with a septum is not completely airtight air moisture permeates through the rubber closure

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Similarly, data from the saline injection groups in both trials were pooled, comprising 29 teeth from the investigator-initiated study and 34 teeth from the Phase I trial