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Western Blot Total protein content of cell lines or the tumor was extracted by lysis in radioimmunoprecipitation assay buffer containing protease inhibitor cocktail and the protein phosphatase inhibitor, and the total protein concentration was quantified by the BCA (bicinchoninic acid) method

Emam H, Beheiri G, Elsalanty M, Sharawy M

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Modification of the 3 Ts protocol in patients with Erectile Dysfunction A substantial proportion of men with PD present with concomitant ED