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Replace them once they are saturated, so you dont cross contaminate

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DA neurons were identified based on their electrophysiological and pharmacological properties (see Materials and Methods

To further assess the effect of CSE on epithelial barrier function, we treated the A549 cell line, the RPMI2650 human nasal epithelial cell line, the BEAS-2B human bronchial epithelial cell line, and the MLE12 mouse lung epithelial cell line with CSE and measured their transepithelial electrical resistance (TEER)

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