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Anti-mouse or anti-rabbit secondary antibodies (Cell Signaling Technology) HRP-conjugated at 1:5000 dilution were incubated with the membranes for 1 h at room temperature, and visualized using a chemiluminescence system

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The most common physiological substrate is thought to be methylgloxyal, which is notably formed from triose phosphate, either non-enzymically or as an intermediate in the triose-phosphate isomerase reaction (Maiti et al., 1997

The skin gradually heals, revealing a smoother, more even complexion