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The mice were randomly divided into four groups (each containing at least five mice): a natural aging group, a natural aging + FOXO4-DRI group, a D-Gal group, and a D-Gal + FOXO4-DRI group

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Consistency across reconstitution sessions Using a standardised diluent, rather than varying water sources, helps maintain consistency in concentration calculations and reduces one more variable in an already complex experimental setup

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