E. cloni-EXPRESS BL21(DE3)Chemically Competent Cells BAC-Optimized Replicator An amber suppressor strain (supE)

An amber suppressor strain (supE) prepared as highly efficient (≥4 × 1010 cfu/µg) electrocompetent cells for phage display library screening

500 ng of uncut plasmid DNA

CircLigase ssDNA Ligase ligates ends of ssDNA in the absence of a complementary sequence

Specifically designed for DNA Buccal Swabs

Exonuclease I completely digested the linear ssDNA oligo

E. cloni-EXPRESS BL21(DE3)Chemically Competent Cells BAC-Optimized Replicator An amber suppressor strain (supE)E. cloni EXPRESS BL21(DE3) Competent Cells Best value for routine protein expression available as Electrocompetent and Chemically Competent Cells. Lucigens E. cloni EXPRESS BL21(DE3) Electrocompetent Cells are the first to offer high efficiency cloning and high level protein expression in the same cell. Cloning efficiencies are increased 25 1,000 fold relative to competitors preparations of BL21 cells (Figure 1), which is essential for construction of