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cDNA was synthesized from 1 g of purified RNA using HiScript II Q RT SuperMix (Vazyme) and subjected to quantitative real-time PCR (q-PCR) for analyzing the relative mRNA levels of specific genes with proper primers using ChamQ Universal SYBR qPCR Master Mix (Vazyme)

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Superfamily of plant, fungal and bacterial peroxidases

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275 In chondrocytes, miR-140 is primarily regulated by SOX9 and targets multiple genes involved in bone and cartilage development, differentiation, and proliferation