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Determine total peptide needed first: if your protocol calls for 5mg daily for 30 days, you need 5 30 = 150mg total

Poyan Mehr, A

Behavioural Brain Research 218: 57-63

Inject diluent down the inside wall of the vial do not aim directly at the lyophilised powder

In our hands, S458D did not disrupt DDD-COP1 assembly or architecture by negative-stain EM, although we cannot exclude functional effects in cells or more subtle conformational changes