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Plasma was collected and stored at 80 C and the PBMC layer was collected into a new 50-ml Falcon tube, resuspended to 50 ml with PBS and centrifuged at 800 g for 5 min at room temperature

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Treatment of Tyramine Side Effects: Regardless of the initial severity, the pressor episodes evoked by tyramine are clinically transient and resolve spontaneously within 2 to 4 hours as the displaced norepinephrine is naturally cleared from the synaptic cleft and metabolized (Gillman, 2017b)