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Urinary bladder hypersensitivity and dysfunction in female mice following early life and adult stress

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253 , F513F521 (1987)

Immunoblot of indicated proteins in HeLa cells treated with Erastin (5 M) and co-treated with either GSH (10 mM), GSHee (10 mM), Trolox (50 uM) or Ferrostatin-1 (5 uM) for 48 hours

Pen Dosage Chart Dosage & Protocols Variations Standard Research Protocol Dose: 0.1 0.3 mg (= 412 clicks) Duration: 2 4 weeks Frequency: Once daily, prior to rest phase Cycle Interval: 2 weeks off before repeating Goal / Description: Baseline protocol used in sleep architecture and circadian rhythm research models

Generation of monoclonal antibodies against mouse Fsp1 Female Wistar rats (RjHan:Wi, age 160 days) were immunized subcutaneously and intraperitoneally with a mixture of 70 g recombinant C-terminal-His tagged full-length mouse Fsp1 protein in 200 l PBS, 5 nmol CpG2006 (TIB MOLBIOL), and 200 l Incomplete Freunds adjuvant (Sigma-Aldrich)