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NHEK was cultured in KBM2 supplemented with 0.4% bovine pituitary extract, 0.33 g ml 1 hydrocortisone, 0.39 g ml 1 epinephrine, 10 g ml 1 transferrin and 1.8 mM CaCl 2 , with or without hHGF or dimeric macrocycle
Abbreviations ALT: Alanine aminotransferase AST: Aspartate aminotransferase BMI: Body mass index CAP: Controlled attenuation parameter FBS: Fasting blood sugar HbA1c: hemoglobin A1c HDL: High-density lipoprotein IRI: Immunoreactive insulin LDL: Low-density lipoprotein LSM: Liver stiffness measurement NAFLD: Nonalcoholic fatty liver disease NASH: Nonalcoholic steatohepatitis NEFA: Non-esterified fatty acid UMIN: University Hospital Medical Information Network VCTE: Vibration-controlled transient elastography References Day CP

55 The SURMOUNT Program includes four global phase 3, randomized, placebo-controlled clinical trials with tirzepatide administered subcutaneously once weekly in conjunction with lifestyle intervention.47, 48, 49 There was a substantial degree of weight reduction with 10 mg and 15 mg doses of 18.420.9% in persons with obesity or preobesity (without diabetes), with improvements in cardiometabolic risk factors (Table 2).47, 48, 49 Lower bodyweight reductions of 12.8% and 14.7% with 10 mg and 15 mg doses of tirzepatide, respectively, were observed in persons with preobesity or obesity and T2DM
GHK-Cu (Copper Tripeptide-1) is a naturally occurring copper-binding peptide complex studied for its role in tissue remodeling, collagen signaling, cellular repair pathways, and sin regeneration mechanisms
It holds no official status as a medicinal product or therapeutic agent within European regulatory frameworks