Chromatin was diluted (without touching the pellet) in 200 l of RIPA buffer (50 mM Tris-Cl (pH = 8), 150 mM NaCl, 5 mM EDTA, 1 % NP-40, 0.5 % sodium deoxycholate, 0.1% SDS, 1x protease and 1x phosphatase inhibitors, followed by addition of 100 l of 3Laemmli sample buffer
Therefore, acetylation of p53 at K98 is essential for p53-mediated ferroptosis (Pegg, 2008
Stick to recommended protocols rather than assuming higher doses will produce faster results
J.HoggP
Funding This work was supported by National Natural Science Foundation of China (81971956 and U1704189) and Natural Science Foundation of Henan Province of China (212300410070)
Composition of L-Glutathione Per 1 capsule: L-Glutathione / GSH (50 mg), L-Glutathione (50 mg), Silicon Dioxide, Microcrystalline Cellulose, Vegetable Capsule, Vegetable Magnesium Stearate