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The plasmid was linearized by the restriction enzyme (Thermo Fisher, Waltham, MA, USA) and used as the template to produce mRNA with T7 High Yield RNA Transcription kit (#DD4202, Vazyme)

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Note: A 4.5 mg weekly dose draws about 45 units (0.45 mL) roughly half the reconstituted vial

For researchers studying body composition preservation, our muscle growth and recovery guide covers compounds that may complement metabolic interventions

Ann Clin Transl Neurol (2018) 5(10):121121

Some people with vitamin B12 deficiency may experience mood changes, such as depression, anxiety, and irritability