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The pipette-to-bath DC resistance of patch electrodes ranged from 4.0 to 5.2, and were filled with a internal solution of the following composition (in mM): 25 CsCl, 100 CSCH3O3S, 10 phosphocreatine, 0.4 EGTA, 10 HEPES, 2 MgCl 2 , 0.4 Mg-ATP, and 0.04 Na-GTP
Weeks 5 to 8 The first photographable changes show up
When NNMT activity is inhibited, these methyl donors become available for other methylation reactions in the body
The doses of Dihexa and Wortmannin were selected based on a previous study [14,17,18,19]