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Twenty-four hours before each experiment, media were replaced with 750 L fresh media containing 1 mM HEPES (Gibco/Fisher Scientific, Cat# 15-630-080)

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Molecular weight: 403.92 Da

Footnotes Manuscript source: Invited manuscript Specialty type: Gastroenterology and hepatology Country of origin: Argentina Peer-review report classification Grade A (Excellent): 0 Grade B (Very good): B, B Grade C (Good): C, C Grade D (Fair): 0 Grade E (Poor): 0 Conflict-of-interest statement: No conflicts of interest, financial or otherwise, are declared by the authors

Electrolytes, protein, and adequate minerals (especially calcium, magnesium, and vitamin D) are vital for tissue repair